ABSTRACT
In this study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using the extract of Hyssops officinalis L. via greenmethod and confirmed by transmission electron microscopy, field emission scanning electron microscopy, X-ray powderdiffraction and Fourier transforms infrared spectroscopy techniques. In the in vivo section, the anti-angiogenesis and antiinflammatory properties of the NPs were evaluated by the chorioallantoic membrane (CAM) assay and mouse paw edematest (induced by carrageenan), respectively. In the in vitro section, changes in the expression of angiogenesis genes (VEGFand VEGFR) and inflammatory genes (IL-1B and IL-10) were investigated by real-time quantitative polymerase chainreaction technique. In order to evaluate the cytotoxicity of ZnO-NPs, 3-5, 4-dimethylthiazol-2-yl) -5, 2-tetrazolium bromide(MTT) test was used on MDA-MB231 breast adenocarcinoma cell line. The results of the CAM assay showed that theZnO-NPs significantly reduced the number and length of blood vessels, as well as the size and weight of the embryos.Evaluation of mouse paw edema showed that the NPs are able to decrease inflammation. Changes in the expression patternof VEGF and VEGFR genes in MCF7 cells showed that the NPs have inhibitory effect on the expression of both genes.Expression levels of IL-10 and IL-1B genes also increased and decreased, respectively. The MTT test showed that the NPhave the ability to decrease breast cancer cells. In conclusion, our results confirm that the ZnO-NPs synthesized by greenmethod have promising anti-cancer properties.